KMID : 0606920210290030303
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Biomolecules & Therapeutics 2021 Volume.29 No. 3 p.303 ~ p.310
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Kaempferol Regulates the Expression of Airway MUC5AC Mucin Gene via I¥êB¥á-NF-¥êB p65 and p38-p44/42-Sp1 Signaling Pathways
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Li Xin
Jin Fengri Lee Hyun-Jae Lee Choong-Jae
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Abstract
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In the present study, kaempferol, a flavonoidal natural compound found in Polygonati Rhizoma, was investigated for its potential effect on the gene expression and production of airway MUC5AC mucin. A human respiratory epithelial NCI-H292 cells was pretreated with kaempferol for 30 min and stimulated with epidermal growth factor (EGF) or phorbol 12-myristate 13-acetate (PMA), for the following 24 h. The effect on PMA-induced nuclear factor kappa B (NF-¥êB) signaling pathway or EGF-induced mitogen-activated protein kinase (MAPK) signaling pathway was investigated. Kaempferol suppressed the production and gene expression of MUC5AC mucins, induced by PMA through the inhibition of degradation of inhibitory kappa B¥á (I¥êB¥á), and NF-¥êB p65 nuclear translocation. Also, kaempferol inhibited EGF-induced gene expression and production of MUC5AC mucin through regulating the phosphorylation of EGFR, phosphorylation of p38 MAPK and extracellular signal-regulated kinase (ERK) 1/2 (p44/42), and the nuclear expression of specificity protein-1 (Sp1). These results suggest kaempferol regulates the gene expression and production of mucin through regulation of NF-¥êB and MAPK signaling pathways, in human airway epithelial cells.
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KEYWORD
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MUC5AC, Pulmonary mucin, Kaempferol
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